Ultrasensitive detection of protein – membrane association is demonstrated via second harmonic scattering.

In this highly collaborative study, the ultrasensitive detection of protein binding to liposomes is shown via second harmonic scattering technique. The binding of Perfringolysin O (PFO), a pore-forming toxin, having a high membrane composition selectivity for its membrane is utilized, and a dissociation constant of 10^-12 M is determined for its binding to cholesterol-rich membranes.

An article exploring the specific-ion effects on local electric fields in aqueous electrolyte solutions is published at JPCB.

A collaborative, multi-perspective investigation gives rise to the molecular level detailed picture of ions’ influence on the vibrational stark effect of various molecular probes. Salts containing well-hydrated cations like Mg2+ or Li+ led to the most notable Stark shift for the carbonyl group, while poorly hydrated cations (Cs+) had the greatest impact on the nitrile groups.

An unforeseen feature of water as a biological fluid is demonstrated. Hyaluronan, a highly hydrated natural biopolymer, has an impact on the structure of water molecules in its extended hydration shells.

In this work, we explored the extended hydration shell structure of hyaluronan in aqueous solutions . Hyaluronan is a highly hydrated biopolymer found in the extracelular environment, such as synovial fluid between joints. Angle-resolved fs-ESHS measurements and nonlinear optical modeling show that hyaluronan behaves like a flexible chain surrounded by extended shells of orientationally correlated water. The spatial extent of the hydration shell is determined via temperature-dependent measurements and can reach up to 475 nm. (read more…)